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Title   대장선종 - 선암연속체 규명을 위한 선종조직 배양상의 문제점 ( Initial Resulta of Adenoma Culture for Adenoma - Carcinoma Continuum in the Colorectal Tumor )
Publicationinfo   1992 Jan; 024(02): 218-227.
Key_word   Tissue culture, Colorectal adenomas, Sequence
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Abstract   Tissue culture of the colorectal adenomas observing the sequence of adenoma-carcinoma continuum was tried using 2 cases from familial polyposis coli and 4 cases from adenomas with synchronous adenocarcinomas known as risk factors of adenocarcinoma. 9 cell lines were established through 1 to 3 subculture(s) from ll adenomas. The growth patterns were not different in respect to size, histopathological type, degree of dysplasia, and ploidy. Either anchorage- independent culture in the agar media or tumorigenicity in the nude mice was not found. 4 principal bile acids in vivo, e.g. cholic acid, chenodeoxycholic acid, deoxycholic acid, and litho- cholic acid, of 10 7M or 10-5M were added into MEM 2 media for the identification of prolif- erative effect. The colonies increased to 1.86 folds in the 10M chendexoycholic acid and 1.19 folds in the 10 'M lithocholic acid, but the colonies changed into reticular destruction due to cytolysis in the all wells of 4 bile acids after 2 weeks. 10' cells from second subcultivated adenocarcinoma was cocultured with adenoma cells from the same host. The adenocarcinoma cells were proliferated destroying the adenoma cells completely within 48 hours. The cultivated adenoma cells showed low biological aggressiveness and proliferation, which suggested the retarded growth and selectiveness among adenomas in the colorectal adenoma- adenocarcinoma sequence. Bile acids might affect early stages of neoplasms. Selective predominance of adenocarcinoma comparing adenoma resulted from coculture of adenoma and adenocarcinoma cells.
저 자   김진천(Jin Cheon Kim),박규연(Kyoo Yeon Park),박건춘(Kun Choon Park),이재담(Jae Dam Lee)